Description

This experiment was conducted to determine the protective effects of methanolic extract of Digera muricata ( L.) Mart. on the acrylamide induced injuries in liver tissues of rats at the histology and biochemical levels. Forty eight healthy female albino rats, weighting 190-200 g, were provided by the Animal House of National Institute of Health (NIH) Islamabad and were maintained at the Primate Facility maintained at Quaid-i-Azam University, Islamabad. These rats were divided into eight groups with six rats in each group. Among them group I remained as control, while group II was treated with DMSO 5.0 ml/kg B.w.(Body weight) orally once a day for five weeks. Group III was given 200 mg/kg B.w. of methanolic extract of Digera muricata dissolved in DMSO once a day for five weeks. Rest of the rats were divided into five groups and were treated with aqueous solution of acrylamide 6 mg/kg B.w. intraparetoneally once a day for two weeks. Group IV was sacrificed after the acrylamide treatment to collect the serum and liver. Group V remained untreated as such for the rest of the experiment. Group VI, VII and VIII were given 100, 150 and 200 mg/kg B.w. methanolic extract of Digera muricata